Synergistic effects of a combined exposure to herbicides and an insecticide in Hyla versicolor

By: , and 
Edited by: Gregory L. LinderSherry K. KrestDonald W. Sparling, and Edward E. Little


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Combinations of the herbicides atrazine and metolachlor and the insecticide chlorpyrifos were tested under both laboratory and field conditions to determine their individual and combined effects on amphibian populations. In the lab Hyla versicolor tadpoles experienced 100% mortality when exposed to a high combination of the pesticides (2.0 mg/L atrazine, 2.54 mg/L metolachlor, 1.0 mg/L chlorpyrifos) whereas low concentrations of the pesticides (0.2 mg/L atrazine, 0.25 mg/L metolachlor, 0.1 mg/L chlorpyrifos) or high concentrations of either herbicides or insecticide alone caused lethargy, reduced growth and delayed metamorphosis but no significant mortality. In the field high herbicide, low insecticide and low herbicide, low insecticide mixtures significantly reduced amphibian populations compared to controls but in the low herbicide, low insecticide wetlands amphibian populations were able to recover through recruitment by the end of the season.

Additional publication details

Publication type Book chapter
Publication Subtype Book Chapter
Title Synergistic effects of a combined exposure to herbicides and an insecticide in Hyla versicolor
Series number 1443
Year Published 2003
Language English
Publisher ASTM International
Publisher location West Conshohocken, PA
Contributing office(s) Patuxent Wildlife Research Center
Description viii, 282
Larger Work Type Book
Larger Work Subtype Other Government Series
Larger Work Title Multiple stressor effects in relation to declining amphibian populations
First page 111
Last page 129