Minimizing microbial contamination of sperm samples

Edited by: Terrence R. Tiersch and Christopher C. Green



Taken from the Methods section: With the collection and translocation of gametes from aquatic species, a potential hazard exists for microbial transfer. Contamination of semen can occur during collection, processing, storage, and transport. Some preventative measures are described below for limiting the spread and amplification of microorganisms such as bacteria, viruses, fungi, mycoplasmas, and parasites. Generally, sanitation during collection is essential. Materials and equipment used to freeze semen should be sterile. Following good practice guidelines for handling and processing samples collected for freezing is especially important for non-domestic animals where disease-free status cannot be guaranteed and unsophisticated technology is used (Russell et al. 1977).

Additional publication details

Publication type Book chapter
Publication Subtype Book Chapter
Title Minimizing microbial contamination of sperm samples
Edition 2
Year Published 2011
Language English
Publisher Cryopreservation in Aquatic Species, 2nd edition
Publisher location Baton Rouge, LA
Contributing office(s) National Wetlands Research Center
Description 4 p.
Larger Work Type Book
Larger Work Subtype Other Government Series
Larger Work Title Cryopreservation in Aquatic Species
First page 684
Last page 687