Retention behavior of long chain quaternary ammonium homologues and related nitroso-alkymethylamines

Journal of Chromatography



Several chromatographic methods have been utilized to study the retentionbehavior of a homologous series of n-alkylbenzyldimethylammonium chlorides (ABDAC) and the corresponding nitroso-n-alkylmethylamines (NAMA). Linear correlation of the logarithmic capacity factor (k') with the number of carbons in the alkyl chain provides useful information on both gas chromatographic (GC) and high-performance liquid chromatographich (HPLC) retention parameters of unknown components. Under all conditions empolyed, GC methodology has proved effective in achieving complete resolution of the homologous mixture of NMA despite its obvious inadequacy in the separation of E-Z configurational isomers. Conversely, normal-phase HPLC on silica demonstrates that the selectivity (a) value for an E-Z pair is much higher than that for an adjacent homologous pair. In the reversed-phase HPLC study, three different silica-based column systems were examined under various mobile phase conditions. The extent of variation in k' was found to be a function of the organic modifier, counter-ion concentration, eluent pH, nature of counter-ion, and the polarity and type of stationary phase. The k'—[NaClO4] profiles showed similar trends between the ABDAC and the NAMA series, supporting the dipolar electronic structures of the latter compounds. Mobile phase and stationary phase effects on component separation are described. The methodology presented establishes the utility of HPLC separation techniques as versatile analytical tools for practical application.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Retention behavior of long chain quaternary ammonium homologues and related nitroso-alkymethylamines
Series title Journal of Chromatography
DOI 10.1016/S0021-9673(01)81321-2
Volume 324
Issue 2
Year Published 1985
Language English
Publisher Elsevier
Publisher location Amsterdam, Netherlands
Contributing office(s) Upper Midwest Environmental Sciences Center
Description 22 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Chromatography
First page 209
Last page 230