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Non-lethal sampling for the detection of Myxobolus cerebralis in asymptomatic rainbow trout

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Abstract

We have described in previous reports (Schill et al., 1998) the development of a polymerase chain reaction (PCR) amplification of 18S ribosomal RNA for the detection of Myxozoan parasites. Oligonucleotide primers were developed by multiple alignment of Myxozoan sequence information and analysis by a custom-written computer program (PRIM). Candidate pairs of primer sequences were then analyzed for specificity by BLAST (Basic Local Alignment Search Tool). From these, a set of promising primers (MYXFWD and MYXREV) was chosen for further testing. These were chosen because they should direct detection of a number of Myxozoan species (Table 1). PCR using MXYFWD and MYXREV proved to be robust and relatively free of artifact products. Further, we were able to routinely detect Myxobolus cerebralis in fish tissues (Figure 1).

Additional publication details

Publication type Book
Publication Subtype Conference publication
Title Non-lethal sampling for the detection of Myxobolus cerebralis in asymptomatic rainbow trout
Year Published 1999
Language English
Publisher Whirling Disease Foundation
Publisher location Bozeman, MT
Contributing office(s) Leetown Science Center
Description p. 235-236
Larger Work Title National Whirling Disease Symposium 1999 Missoula, MT
First page 235
Last page 236
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