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Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state

Environmental Toxicology and Pharmacology

By:
, , , and
DOI:10.1016/j.etap.2006.03.005

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Abstract

Apoptosis, or programmed cell death, has been proposed as a biomarker for environmental contaminant effects. In this work, we test the hypothesis that in vitro assays of apoptosis are sensitive indicators of immunological effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophils. Apoptosis, necrosis, and viability as well as the related indicators F-actin levels, and active thiol state were measured in purified human neutrophils after treatment with contaminants. Effective concentrations observed were 0.3 μM (60 μg/L) mercury, 750 μg/L Aroclor 1254, and 50 μM (14,500 μg/L) hexachlorocylcohexanes. Concentrations of contaminants that induced apoptosis also decreased cellular F-actin levels. Active thiols were altered by mercury, but not organochlorines. Comparison of these data with levels of contaminants reported to be threats to human health indicate neutrophil apoptosis is a sensitive indicator of mercury toxicity.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state
Series title:
Environmental Toxicology and Pharmacology
DOI:
10.1016/j.etap.2006.03.005
Volume:
22
Issue:
2
Year Published:
2006
Language:
English
Publisher:
Elsevier
Contributing office(s):
Great Lakes Science Center
Description:
10 p.
First page:
179
Last page:
188
Online Only (Y/N):
N
Additional Online Files (Y/N):
N