Mercury in water and biomass of microbial communities in hot springs of Yellowstone National Park, USA

Applied Geochemistry
By: , and 

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Abstract

Ultra-clean sampling methods and approaches typically used in pristine environments were applied to quantify concentrations of Hg species in water and microbial biomass from hot springs of Yellowstone National Park, features that are geologically enriched with Hg. Microbial populations of chemically-diverse hot springs were also characterized using modern methods in molecular biology as the initial step toward ongoing work linking Hg speciation with microbial processes. Molecular methods (amplification of environmental DNA using 16S rDNA primers, cloning, denatured gradient gel electrophoresis (DGGE) screening of clone libraries, and sequencing of representative clones) were used to examine the dominant members of microbial communities in hot springs. Total Hg (THg), monomethylated Hg (MeHg), pH, temperature, and other parameters influential to Hg speciation and microbial ecology are reported for hot springs water and associated microbial mats.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Mercury in water and biomass of microbial communities in hot springs of Yellowstone National Park, USA
Series title Applied Geochemistry
DOI 10.1016/j.apgeochem.2006.08.004
Volume 21
Issue 11
Year Published 2006
Language English
Publisher Elsevier
Contributing office(s) Toxic Substances Hydrology Program
Description 12 p.
First page 1868
Last page 1879