Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology

Applied and Environmental Microbiology
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Abstract

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem. Copyright ?? 2008, American Society for Microbiology. All Rights Reserved.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology
Series title Applied and Environmental Microbiology
DOI 10.1128/AEM.01303-08
Volume 74
Issue 24
Year Published 2008
Language English
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Applied and Environmental Microbiology
First page 7828
Last page 7831