Mercury distribution and lipid oxidation in fish muscle: Effects of washing and isoelectric protein precipitation

Journal of Agricultural and Food Chemistry
By: , and 

Links

Abstract

Nearly all the mercury (Hg) in whole muscle from whitefish (Coregonus clupeaformis) and walleye (Sander vitreus) was present as methyl mercury (MeHg). The Hg content in whole muscle from whitefish and walleye was 0.04–0.09 and 0.14–0.81 ppm, respectively. The myofibril fraction contained approximately three-fourths of the Hg in whitefish and walleye whole muscle. The sarcoplasmic protein fraction (e.g., press juice) was the next most abundant source of Hg. Isolated myosin, triacylglycerols, and cellular membranes contained the least Hg. Protein isolates prepared by pH shifting in the presence of citric acid did not decrease Hg levels. Addition of cysteine during washing decreased the Hg content in washed muscle probably through the interaction of the sulfhydryl group in cysteine with MeHg. Primary and secondary lipid oxidation products were lower during 2 °C storage in isolates prepared by pH shifting compared to those of washed or unwashed mince from whole muscle. This was attributed to removing some of the cellular membranes by pH shifting. Washing the mince accelerated lipid peroxide formation but decreased secondary lipid oxidation products compared to that of the unwashed mince. This suggested that there was a lipid hydroperoxide generating system that was active upon dilution of aqueous antioxidants and pro-oxidants.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Mercury distribution and lipid oxidation in fish muscle: Effects of washing and isoelectric protein precipitation
Series title Journal of Agricultural and Food Chemistry
DOI 10.1021/jf202411p
Volume 59
Issue 20
Year Published 2011
Language English
Publisher ACS
Contributing office(s) Mercury Research Laboratory, Toxic Substances Hydrology Program
Description 8 p.
First page 11050
Last page 11057
Google Analytic Metrics Metrics page
Additional metadata about this publication, not found in other parts of the page is in this table