Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis)

Conservation Genetics Resources
By: , and 

Links

Abstract

Blackfishes (Esociformes: Esocidae: Dallia), small fishes with relictual distributions, are unique in being the only primary freshwater fish genus endemic to Beringia. Although the number of species of Dallia is debated, disjunct populations and distinct mitochondrial divisions that predate the end of the last glacial maximum are apparent. We developed sixteen polymorphic microsatellites from the Alaska blackfish (Dallia pectoralis) to study genetic diversity in Dallia. Genotypes from two populations, Denali (n = 31) and Bethel (n = 35), demonstrated the usefulness of the loci for population-level investigation. Observed and expected heterozygosity averaged 18.6 and 19.8 % in Denali and 61.1 and 63.7 % in Bethel. Number of alleles at each locus averaged 3.50 in Denali and 9.63 in Bethel. The observed signature of variability and structuring between populations is consistent with mitochondrial data.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis)
Series title Conservation Genetics Resources
DOI 10.1007/s12686-013-0091-6
Volume 6
Issue 2
Year Published 2014
Language English
Publisher Springer
Contributing office(s) Alaska Science Center
Description 3 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Conservation Genetics Resources
First page 349
Last page 351