Development of ten microsatellite loci in the invasive giant African land snail, Achatina (=Lissachatina) fulica Bowdich, 1822

Conservation Genetics Resources
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Abstract

A suite of tetra-nucleotide microsatellite loci were developed for the invasive giant African land snail, Achatina (=Lissachatina) fulica Bowdich, 1822, from Ion Torrent next-generation sequencing data. Ten of the 96 primer sets tested amplified consistently in 30 snails from Miami, Florida, plus 12 individuals representative of their native East Africa, Indian and Pacific Ocean regions. The loci displayed moderate levels of allelic diversity (average 5.6 alleles/locus) and heterozygosity (average 42 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring among regional samples. The invasive A. fulica can cause extensive damage to important food crops and natural resources, including native flora and fauna. The loci characterized here will be useful for determining the origins and tracking the spread of invasions, detecting fine-scale spatial structuring and estimating demographic parameters.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development of ten microsatellite loci in the invasive giant African land snail, Achatina (=Lissachatina) fulica Bowdich, 1822
Series title Conservation Genetics Resources
DOI 10.1007/s12686-014-0332-3
Volume 7
Issue 1
Year Published 2015
Language English
Publisher Springer
Contributing office(s) Leetown Science Center
Description 2 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Conservation Genetics Resources
First page 201
Last page 202
Online Only (Y/N) N
Additional Online Files (Y/N) N