Characterization of a novel hepadnavirus in the white sucker (Catostomus commersonii) from the Great Lakes Region of the USA

Journal of Virology
By: , and 

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Abstract

The white sucker Catostomus commersonii is a freshwater teleost often utilized as a resident sentinel. Here, we sequenced the full genome of a hepatitis B-like virus that infects white suckers from the Great Lakes Region of the USA. Dideoxysequencing confirmed the white sucker hepatitis B virus (WSHBV) has a circular genome (3542 bp) with the prototypical codon organization of hepadnaviruses. Electron microscopy demonstrated that complete virions of approximately 40 nm were present in the plasma of infected fish. Compared to avi- and orthohepadnaviruses, sequence conservation of the core, polymerase and surface proteins was low and ranged from 16-27% at the amino acid level. An X protein homologue common to the orthohepadnaviruses was not present. The WSHBV genome included an atypical, presumptively non-coding region absent in previously described hepadnaviruses. Phylogenetic analyses confirmed WSHBV as distinct from previously documented hepadnaviruses. The level of divergence in protein sequences between WSHBV other hepadnaviruses, and the identification of an HBV-like sequence in an African cichlid provide evidence that a novel genus of the family Hepadnaviridae may need to be established that includes these hepatitis B-like viruses in fishes. Viral transcription was observed in 9.5% (16 of 169) of white suckers evaluated. The prevalence of hepatic tumors in these fish was 4.9%, of which only 2.4% were positive for both virus and hepatic tumors. These results are not sufficient to draw inferences regarding the association of WSHBV and carcinogenesis in white sucker.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Characterization of a novel hepadnavirus in the white sucker (Catostomus commersonii) from the Great Lakes Region of the USA
Series title Journal of Virology
DOI 10.1128/JVI.01278-15
Volume 89
Issue 23
Year Published 2015
Language English
Publisher American Society for Microbiology
Contributing office(s) Leetown Science Center, Contaminant Biology Program
Description 11 p.
First page 11801
Last page 11811
Country United States
Other Geospatial Great Lakes Region
Online Only (Y/N) N
Additional Online Files (Y/N) N