The fractionation of humic acids from natural water systems

Journal of Research of the U.S. Geological Survey
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Abstract

Humic acids, the most abundant organic components of natural water systems, are complex mixtures of molecular aggregates of different chemical and physical properties. The first step in the study of such a mixture is the fractionation of the mixture. The most common approach with humic acids is to attempt to obtain a molecular weight fractionation by gel-permeation chromatography. However, since the preponderance of evidence indicates that the components of humic acid do not fulfill the basic criteria of uniform shape and chemical structure necessary for obtaining a molecular fractionation on gel-permeation media, molecular weight distributions in humic acids cannot be
evaluated by this method. A fractionation dependent upon chemical structure can be obtained by the manipulation of elution conditions on
a gel-permeation column. This procedure provides a beginning in the isolation and identification of discrete components of humic acids.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title The fractionation of humic acids from natural water systems
Series title Journal of Research of the U.S. Geological Survey
Volume 1
Issue 3
Year Published 1973
Language English
Publisher U.S. Geological Survey
Publisher location Reston, VA
Description 6 p.
First page 361
Last page 366
Online Only (Y/N) N
Additional Online Files (Y/N) N
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