Development, characterization, and use of monoclonal and polyclonal antibodies against the myxosporean, Ceratomyxa shasta

Journal of Eukaryotic Microbiology
By: , and 

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Abstract

Both monoclonal and polyclonal antisera were produced against Ceratomyxa shasta. Ascites containing trophozoites of the parasite was collected from infected fish and used as antigen for immunization of mice. The resulting monoclonal antibodies reacted specifically with trophozoite and sporoblast stages but did not react with C. shasta spores by either indirect fluorescent antibody techniques or in Western blots. This indicates that some C. shasta antigens are specific to certain life stages of the parasite. Polyclonal antiserum was produced in a rabbit by injecting a spore protein electro-eluted from an SDS-polyacrylamide gel. This antiserum reacted with both trophozoites and spores by indirect fluorescent antibody techniques and in Western blots. All antisera were tested for cross-reactivity to trout white blood cells, a contaminant of the ascites, and to other myxosporea. Two monoclonal antibodies reacted with white blood cells and myxosporea of the genera Sphaerospora and Myxobilatus. One hybridoma produced antibodies of high specificity for C. shasta pre-spore stages. This is the first report of a monoclonal antibody produced against a myxosporean parasite.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development, characterization, and use of monoclonal and polyclonal antibodies against the myxosporean, Ceratomyxa shasta
Series title Journal of Eukaryotic Microbiology
DOI 10.1111/j.1550-7408.1989.tb05534.x
Volume 36
Issue 4
Year Published 1989
Language English
Publisher Wiley
Description 5 p.
First page 397
Last page 401