Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling

Deep-Sea Research Part II: Topical Studies in Oceanography
By: , and 

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Abstract

Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be standardized, random, and allowed the georeferencing of all sampled colonies. Clonal lineages discriminated through their Multi-Locus Genotypes (MLG) at 6–7 microsatellite markers could thus be mapped to assess the level of clonality and the spatial spread of clonal lineages. High values of clonal richness were observed for both species across all sites suggesting a limited occurrence of clonality, which likely originated through fragmentation. Additionally, spatial autocorrelation analysis underlined the possible occurrence of fine-grained genetic structure in several populations of both L. pertusa and M. oculata. The two cold-water coral species examined had contrasting patterns of connectivity among canyons, with among-canyon genetic structuring detected in M. oculata, whereas L. pertusa was panmictic at the canyon scale. This study exemplifies that a standardized, random and georeferenced sampling strategy, while challenging, can be applied in the deep sea, and associated benefits outlined here include improved estimates of fine grained patterns of clonality and dispersal that are comparable across sites and among species.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling
Series title Deep-Sea Research Part II: Topical Studies in Oceanography
DOI 10.1016/j.dsr2.2015.11.013
Volume 145
Year Published 2017
Language English
Publisher Elsevier
Contributing office(s) Leetown Science Center
Description 11 p.
First page 120
Last page 130
Other Geospatial Bay of Biscay
Online Only (Y/N) N
Additional Online Files (Y/N) N