Analysis of the nucleoprotein gene identifies three distinct lineages of viral haemorrhagic septicemia virus within the European marine environment
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Abstract
A ribonuclease (RNase) protection assay (RPA) has been used to detect nucleotide sequence variation within the nucleoprotein gene of 39 viral haemorrhagic septicaemia virus (VHSV) isolates of European marine origin. The classification of VHSV isolates based on RPA cleavage patterns permitted the identification of ten distinct groups of viruses based on differences at the molecular level. The nucleotide sequence of representatives of each of these groupings was determined and subjected to phylogenetic analysis. This revealed grouping of the European marine isolates of VHSV into three genotypes circulating within distinct geographic areas. A fourth genotype was identified comprising isolates originating from North America. Phylogenetic analyses indicated that VHSV isolates recovered from wild caught fish around the British Isles were genetically related to isolates responsible for losses in farmed turbot. Furthermore, a relationship between naturally occurring marine isolates and VHSV isolates causing mortality among rainbow trout in continental Europe was demonstrated.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Analysis of the nucleoprotein gene identifies three distinct lineages of viral haemorrhagic septicemia virus within the European marine environment |
| Series title | Virus Research |
| DOI | 10.1016/S0168-1702(99)00056-8 |
| Volume | 63 |
| Issue | 1-2 |
| Year Published | 1999 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 10 p. |
| First page | 35 |
| Last page | 44 |
| Country | Ireland, United Kingdom |
| Other Geospatial | British Isles |
| Google Analytic Metrics | Metrics page |