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Ichthyophonus parasite phylogeny based on ITS rDNA structure prediction and alignment identifies six clades, with a single dominant marine type

Diseases of Aquatic Organisms

By:
, ORCID iD , ORCID iD , , and ORCID iD
https://doi.org/10.3354/dao03017

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Abstract

Despite their widespread, global impact in both wild and cultured fishes, little is known of the diversity, transmission patterns, and phylogeography of parasites generally identified as Ichthyophonus. This study constructed a phylogeny based on the structural alignment of internal transcribed spacer (ITS) rDNA sequences to compare Ichthyophonus isolates from fish hosts in the Atlantic and Pacific oceans, and several rivers and aquaculture sites in North America, Europe, and Japan. Structure of the Ichthyophonus ITS1–5.8S–ITS2 transcript exhibited several homologies with other eukaryotes, and 6 distinct clades were identified within Ichthyophonus. A single clade contained a majority (71 of 98) of parasite isolations. This ubiquitous Ichthyophonus type occurred in 13 marine and anadromous hosts and was associated with epizootics in Atlantic herring, Chinook salmon, and American shad. A second clade contained all isolates from aquaculture, despite great geographic separation of the freshwater hosts. Each of the 4 remaining clades contained isolates from single host species. This study is the first to evaluate the genetic relationships among Ichthyophonus species across a significant portion of their host and geographic range. Additionally, parasite infection prevalence is reported in 16 fish species.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Ichthyophonus parasite phylogeny based on ITS rDNA structure prediction and alignment identifies six clades, with a single dominant marine type
Series title:
Diseases of Aquatic Organisms
DOI:
10.3354/dao03017
Volume:
120
Issue:
2
Year Published:
2016
Language:
English
Publisher:
Inter-Research
Contributing office(s):
Western Fisheries Research Center
Description:
16 p.
First page:
125
Last page:
141
Online Only (Y/N):
N
Additional Online Files (Y/N):
N