Differentiation of bacterial feeding nematodes in soil ecological studies by means of arbitrarily primed PCR

Soil Biology and Biochemistry
By: , and 

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Abstract

Arbitrarily-primed polymerase chain reaction (ap-PCR) was used to differentiate closely related bacterial-feeding nematodes of the genera: Caenorhabditis, Acrobeloides, Cephalobus and Zeldia. Average percentage similarity of bands generated by ap-PCR with seven different primers between 14 isolates of Caenorhabditis elegans was ⪢ 90%, whereas between C. elegans, C. briggsae and C. remanei similarity was < 20%. Based on intra- and inter-specific similarity between Caenorhabditis isolates, analysis of Acrobeloides, Cephalobus and Zeldia isolates revealed either similar or different genotypes. Distinct genotypes were verified by morphological analyses. In addition, the genotypes obtained from single egg-derived nematode populations were also obtained from ap-PCR analysis of single worms. Due to the difficulty of identification of soil nematodes, the ap-PCR offers potential as a rapid and reliable technique to assess biodiversity. Ap-PCR will make it feasible, for the first time, to study the ecological interactions of unique nematode genotypes in soil habitats.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Differentiation of bacterial feeding nematodes in soil ecological studies by means of arbitrarily primed PCR
Series title Soil Biology and Biochemistry
DOI 10.1016/0038-0717(93)90208-S
Volume 25
Issue 9
Year Published 1993
Language English
Publisher Elsevier
Contributing office(s) Western Fisheries Research Center
Description 11 p.
First page 1141
Last page 1151