Immunization with viral antigens: Infectious haematopoietic necrosis

Developments in Biological Standardization
Edited by: Paul J. Midtlyng and F. Brown



Infectious haematopoietic necrosis (IHN) is one of the most important viral diseases of salmonids, especially among juvenile fish where losses can be high. For over 20 years, researchers have tested a variety of preparations for control of IHN. Early vaccines consisted of killed virus and were effective when delivered by injection, but too costly to be practical on a large scale. Attenuated vaccines were developed by serial passage in cell culture and by monoclonal antibody selection. These offered excellent protection and were cost-effective, but residual virulence and uncertainty about their effects on other aquatic species made them poor candidates for licensing. Subunit vaccines using part of the IHNV glycoprotein gene cloned into E. coli or into an attenuated strain of A. salmonicida have been tested, appeared safe and were inexpensive. These vaccines were reported to provide some protection when delivered by immersion. Information on the location of antigenic sites on the glycoprotein led to trials using synthetic peptides, but these did not seem to be economically viable. Recently, plasmid vectors encoding the glycoprotein gene under control of a cytomegalovirus promoter were developed for genetic immunization. The constructs were highly protective when delivered by injection, but a more practical delivery system is needed. Thus, while several vaccine strategies have been tried in order to stimulate specific immunity against IHN, more research is needed to develop a commercially viable product for control of this important disease.

Additional publication details

Publication type Conference Paper
Publication Subtype Conference Paper
Title Immunization with viral antigens: Infectious haematopoietic necrosis
Series title Developments in Biological Standardization
ISBN 978-3-8055-6482-3
Volume 90
Year Published 1997
Language English
Publisher PubMed
Contributing office(s) Western Fisheries Research Center
Description 10 p.
Larger Work Type Book
Larger Work Subtype Conference publication
Larger Work Title Fish vaccinology (Developments in Biological Standardization, volume 90)
First page 211
Last page 220
Conference Title Fish Vaccinology
Conference Location Oslo, Norway
Conference Date June 1996