Total protein concentration and diagnostic test results for gray wolf (Canis lupus) serum using Nobuto filter paper strips

Journal of Wildlife Diseases
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Abstract

Nobuto filter paper strips are widely used for storing blood-serum samples, but the recovery of proteins from these strips following rehydration is unknown. Poor recovery of proteins could reduce the concentration of antibodies and antigens and reduce the sensitivity of diagnostic assays. We compared the protein concentration, and its association with test sensitivity, of eluted Nobuto strip samples with paired sera. We collected and froze serum from five gray wolves (Canis lupus) for 8 mo. When thawed, we used a spectrophotometer (absorbance 280 nm) to determine the serum protein concentration for paired sera and Nobuto eluates for each animal in 2-fold serial dilutions. Total protein concentration was similar for both sample storage methods (Nobuto eluates and control sera), except for the undiluted samples in which Nobuto eluates had higher total protein concentrations. Both sample storage methods appear to produce similar results using the SNAP® 4Dx® Test to detect antibodies against pathogens causing Lyme disease, anaplasmosis, and ehrlichiosis as well as antigen for canine heartworm disease.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Total protein concentration and diagnostic test results for gray wolf (Canis lupus) serum using Nobuto filter paper strips
Series title Journal of Wildlife Diseases
DOI 10.7589/2013-07-185
Volume 51
Issue 2
Year Published 2015
Language English
Publisher Wildlife Disease Association
Contributing office(s) Coop Res Unit Leetown
Description 4 p.
First page 475
Last page 478