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A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

Conservation Genetics Resources

By:
ORCID iD , ORCID iD , , and
https://doi.org/10.1007/s12686-017-0812-3

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Abstract

A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes proliferative kidney disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. The limits of detection and quantification were 7 and 100 DNA copies for calibration standards and T. bryosalmonae was reliably detected down to 100 copies in tissue and eDNA samples. The assay presented here is a highly sensitive and quantitative tool for detecting T. bryosalmonae with potential applications for tissue diagnostics and environmental detection.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
Series title:
Conservation Genetics Resources
DOI:
10.1007/s12686-017-0812-3
Edition:
Online First
Year Published:
2017
Language:
English
Publisher:
Springer
Contributing office(s):
Northern Rocky Mountain Science Center