Large-scale dialysis of sample lipids

Chemosphere
By: , and 

Links

Abstract

The use of a semipermeable membrane device (SPMD) for dialysis in an organic solvent phase is an efficient alternative approach to separation of contaminants from large amounts of lipid (up to 50 grams or more) prior to organic chemical analysis. Passive separation of contaminants can be accomplished with a minimum of equipment and a comparatively small volume of solvent. This study examines the effects of factors such as dialytic solvent, lipid type, dialytic solvent:lipid volume ratio, dialysis time, and temperature on the performance of polyethylene SPMDs during lipid-contaminant separations. The experimental conditions for maximal recoveries of organochlorine pesticides and polychlorinated biphenyls with minimal lipid carryover are determined for the examined variables. When the dialytic procedure is optimized, very satisfactory and highly reproducible analyte recoveries can be obtained in a few days while separating > 90% of the lipid material in a single operation.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Large-scale dialysis of sample lipids
Series title Chemosphere
DOI 10.1016/0045-6535(93)90026-2
Volume 26
Issue 11
Year Published 1993
Language English
Publisher Elsevier
Contributing office(s) Columbia Environmental Research Center
Description 14 p.
First page 1993
Last page 2006
Country United States