Phylogenetic techniques in geomicrobiology

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Abstract

Molecular biological techniques have revolutionized the field of geomicrobiology by providing researchers with robust techniques for identifying microorganisms and characterizing microbial communities in a wide variety of environments. These techniques have freed researchers from the constraints of classical culture-based microbiology and allowed the discovery of previously unknown phylogenetic diversity of microorganisms. In this chapter, we discuss the theory, methods, and workflow for applying molecular techniques to identify and characterize microbial populations. Our chapter focuses on SSU rRNA gene-based approaches, guiding the reader from sample collection and gene amplification through bioinformatics and statistical analysis. The workflow presented has been successfully used to identify microbial populations and community dynamics in a wide variety of habitats to understand the interactions between microbes and their environment.

Additional publication details

Publication type Book chapter
Publication Subtype Book Chapter
Title Phylogenetic techniques in geomicrobiology
Chapter Part V: Microbiological Techniques
Sub-chapter 15
DOI 10.1017/9781107707399.015
Year Published 2019
Language English
Publisher Cambridge University Press
Contributing office(s) WMA - Earth System Processes Division
Description 45 p.
Larger Work Type Book
Larger Work Subtype Monograph
Larger Work Title Analytical Geomicrobiology: A Handbook of Instrumental Techniques
First page 360
Last page 404
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