Quantifying suspended-sediment transport is critical for a variety of disciplines related to the management of water resources. However, the number of gauging stations and monitoring networks in most rivers around the world is insufficient to improve understanding of river dynamics and support water resource management decisions. This is mainly due to the high operational costs and intensive labor involved in traditional sediment measurement techniques, especially in sand bed rivers where coarse material varies spatially in the river cross section. Recently, the acoustic surrogate method has received attention as a potentially accurate surrogate technology for estimating suspended-sediment concentrations. In addition, the acoustic surrogate method, through use of acoustic Doppler current profilers (ADCPs), has the advantage of being able to simultaneously measure the flow velocity field and cross-sectional area when moving-boat measurements are performed. In spite of the important advances made in the implementation of this technique, there are no widely-available, free tools for processing the ADCP acoustic signal cross section measurements which include options to extrapolate velocity and sediment in unmeasured ADCP zones and develop calibrations with physical samples. This paper presents a new software called Acoustic Sediment Estimation Toolbox (ASET), which enables the user to develop a calibration between the acoustic signal collected with a down-looking Teledyne RD Instruments ADCP and sediment concentrations determined using traditional sediment sampling techniques. Moreover, ASET software uses dynamic ADCP measurements to estimate the total suspended-sediment transport through a river cross section. The theoretical framework and data processing routines applied by each module in ASET are presented. Finally, a comparison is made between the results obtained by ASET and by traditional methodologies for computing suspended-sediment transport in a large river system (Paraná River, Argentina).