Over the past two decades, extensive monitoring has been conducted in the St. Clair – Detroit River System to describe spatial and temporal patterns of lake sturgeon (Acipenser fulvescens). To characterize spatial patterns in juvenile lake sturgeon (<1000 mm TL) based on survey collections, ‘hot spots’ were identified through optimized hot spot analysis (HSA). This HSA was then interpolated by inverse distance weighted analysis to determine extent of identified ‘hot spots’ and ‘cold spots’. Additionally, habitat variables (i.e., water depth, water velocity, and dominant substrate type) were investigated using a single season occupancy model to determine their influence on juvenile lake sturgeon occupancy probability. In total, 1203 juvenile lake sturgeon were captured across 4197 surveys. Three unique ‘hot spots’ were identified; western Lake Erie, Fighting Island in the Detroit River, and the North Channel in the St. Clair River. Interpolated ‘hot spots’ encompassed 73.1 km² in western Lake Erie, 4.7 km² near Fighting Island, and 6.6 km² in the North Channel. Detection probabilities within ‘hot spots’ ranged from 8.8%–43.4%. No habitat variables significantly predicted juvenile lake sturgeon occupancy. Juvenile lake sturgeon were captured in western Lake Erie where the water depth was >5.1 m and odds of occupancy increased with increased water velocity. Juvenile lake sturgeon in the Detroit and St. Clair River ‘hot spots’ were captured at sites with mean benthic water velocities ranging from 0.20–0.60 m/s and where water depth was >7.3 m. Irrespective of waterbody, 69% of all juveniles were detected over dominant sand and gravel substrates. These results provide valuable insight about juvenile habitat use that can help managers formulate effective conservation and restoration strategies supporting the continued recovery of Great Lakes lake sturgeon.