Four intensive data-collection efforts, intended to represent the spectrum of precipitation events and associated flow conditions, were conducted during 1997 and 1998 in the Taylor Slough Basin, Everglades National Park. Flow velocities were measured by newly developed, portable Acoustic Doppler Velocity meters along three transects bisecting the Taylor Slough Basin from east to west, roughly perpendicular to the centerline axis of the slough as well as a fourth transect along the slough's axis. These meters provided the required levels of accuracy in flow-velocity measurements while enabling the rapid collection of multiple time series of flow data at remote sites. Concurrently, flow measurements were made along bordering road culverts and under L-31W and Taylor Slough bridges. Flows across the study area's boundaries provided net flow of water into the system and transect measurements provided flow data within the basin. Collected data are available through the World Wide Web (http://sofia.usgs.gov/projects/flow_velocity/). The high-water and low-water events corresponded with the highest and lowest flow velocities, respectively. The July 1998 data had lower than expected flow velocities and, in some cases, strong winds reversed flow direction.