Occurrence and distribution of fecal indicator bacteria and gene markers of pathogenic bacteria in Great Lakes tributaries, March-October 2011

Open-File Report 2015-1013
Prepared in cooperation with the Great Lakes Restoration Initiative
By: , and 

Links

Abstract

From March through October 2011, the U.S. Geological Survey (USGS), conducted a study to determine the frequency of occurrence of pathogen gene markers and densities of fecal indicator bacteria (FIB) in 22 tributaries to the Great Lakes. This project was funded as part of the Great Lakes Restoration Initiative (GLRI) and included sampling at 22 locations throughout 6 states that border the Great Lakes.

A total of 177 environmental samples were collected at USGS streamgaging stations during both normal-flow and high-flow conditions and were analyzed by the Michigan Bacteriological Research Laboratory at the USGS Water Science Center in Lansing, Michigan.

Water samples were analyzed for the presence of FIB concentrations (FIB; fecal coliform bacteria, Escherichia coli [E. coli], and enterococci) by using membrane filtration and serial dilution methods. The resulting enrichments from standard culturing of the samples were then analyzed by using polymerase chain reaction (PCR) to determine the occurrence of pathogen gene markers for Shigella species, Campylobacter jejuni and coliSalmonellaspecies, and pathogenic E. coli, including Shiga toxin-producing E. coli (STEC).

Study Area

Additional publication details

Publication type Report
Publication Subtype USGS Numbered Series
Title Occurrence and distribution of fecal indicator bacteria and gene markers of pathogenic bacteria in Great Lakes tributaries, March-October 2011
Series title Open-File Report
Series number 2015-1013
DOI 10.3133/ofr20151013
Year Published 2015
Language English
Publisher U.S. Geological Survey
Publisher location Reston, VA
Contributing office(s) Michigan Water Science Center
Description v, 29 p.
Time Range Start 2011-03-01
Time Range End 2011-10-31
Country United States
Other Geospatial Great Lakes
Online Only (Y/N) Y
Additional Online Files (Y/N) N