Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.)
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Abstract
Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus, was investigated by heat shock, cold shock, hydrostatic pressure, and/or chemicals (cytochalasin A, B, and D). Additionally, efficacy of combined protocols was determined. Heat shock 10 min after fertilization induced triploidy when incubation temperature was 24 C but not when incubation temperature was 31 C. Heat shock of 40–41 C at 4–6 min after fertilization was effective in inducing up to 100% triploidy with hatchability similar to controls. Cold shock at 13 C for 45 min five min after fertilization induced 85–100% triploids. Heat shock and multiple heat shocking were the most effective treatments for the induction of tetraploidy. Two heat treatments of 41 C applied at 65 and 80 min after fertilization for 5 min each produced approximately 80% tetraploidy in hatched fry. Immersion of fertilized eggs in cytochalasin A, B, or D at concentrations up to 10 μg/L applied at various times and durations was ineffective in inducing triploidy or tetraploidy.
Publication type | Article |
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Publication Subtype | Journal Article |
Title | Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.) |
Series title | Journal of the World Aquaculture Society |
DOI | 10.1111/j.1749-7345.1999.tb00875.x |
Volume | 30 |
Issue | 2 |
Year Published | 1999 |
Language | English |
Publisher | World Aquaculture Society |
Contributing office(s) | National Wetlands Research Center |
Description | 7 p. |
First page | 269 |
Last page | 275 |
Google Analytic Metrics | Metrics page |