Some enzymatic properties of brain Acetylcholinesterase from bluegill and channel catfish

Journal of the Fisheries Research Board of Canada
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Abstract

Using a manometric technique an acetylcholinesterase (EC 3.1.1.7, acetylcholine acetyl-hydrolase) was demonstrated in brain tissue from the bluegill, Lepomis macrochirus Rafinesque, and the channel catfish, Ictalurus punctatus (Walbaum). The activities were 19 and 37 μmoles acetylcholine hydrolyzed/milligram protein per hour for the bluegill and channel catfish enzymes, respectively. The optimum substrate concentration for the hydrolysis of acetylcholine was 10 mMfor the enzymes from both species. Generally, the catfish acetylcholinesterase was somewhat more susceptible than the bluegill to the inhibitors tested; however, the bluegill enzyme was more susceptible to inhibition by malathion and malaoxon.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Some enzymatic properties of brain Acetylcholinesterase from bluegill and channel catfish
Series title Journal of the Fisheries Research Board of Canada
DOI 10.1139/f68-059
Volume 25
Issue 4
Year Published 1968
Language English
Publisher NRC Research Press
Contributing office(s) Columbia Environmental Research Center
Description 9 p.
First page 615
Last page 623
Online Only (Y/N) N
Additional Online Files (Y/N) N