Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization

Conservation Genetics Resources
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Abstract

Butler’s Gartersnakes (BGS; Thamnophis butleri) are confined to open and semi-open canopy wetlands and adjacent uplands, habitats under threat of development in Wisconsin. To address issues of species identity and putative hybridization with congeneric snakes, a suite of 18 microsatellite loci capable of cross-species amplification of Plains Gartersnakes (T. radix) and Common Gartersnakes (T. sirtalis) was developed. All loci were polymorphic in BGS with mean number of alleles per locus of 16.11 (range = 3–41) and mean observed heterozygosity of 0.659 (range = 0.311–0.978). Loci amplified efficiently in the congeneric species with high levels of intra- and inter-specific variation. These loci will aid ongoing efforts to effectively identify and manage BGS in Wisconsin.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization
Series title Conservation Genetics Resources
DOI 10.1007/s12686-011-9555-8
Volume 4
Issue 2
Year Published 2012
Language English
Publisher Springer
Contributing office(s) Coop Res Unit Leetown
Description 4 p.
First page 383
Last page 386