Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing

Mitochondrial DNA Part A
By: , and 

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Abstract

Mitochondrial genetic markers have been extensively used to study the phylogenetics and phylogeography of many birds, including seabirds of the order Procellariiformes. Evidence suggests that part of the mitochondrial genome of Procellariiformes, especially albatrosses, is duplicated, but no DNA fragment covering the entire duplication has been sequenced. We sequenced the complete mitochondrial genome of a non-albatross species of Procellariiformes, Puffinus lherminieri (Audubon’s shearwater) using the long-read MinION (ONT) technology. Two mitogenomes were assembled from the same individual, differing by 52 SNPs and in length. The shorter was 19 kb long while the longer was 21 kb, due to the presence of two identical copies of nad6, three tRNA, and two dissimilar copies of the control region (CR). Contrary to albatrosses, cobwas not duplicated. We further detected a complex repeated region of undetermined length between the CR and 12S. Long-read sequencing suggests heteroplasmy and a novel arrangement within the duplicated region, indicating a complex evolution of the mitogenome in Procellariiformes.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing
Series title Mitochondrial DNA Part A
DOI 10.1080/24701394.2018.1484116
Volume 30
Issue 2
Year Published 2019
Language English
Publisher Taylor & Francis
Contributing office(s) Patuxent Wildlife Research Center
Description 8 p.
First page 256
Last page 263